Genome-wide association study of agronomy and morphology and morphology traits in elite spring wheat Germplasm evaluated under salt stress conditions in Egypt

Published Date
November 16, 2021
Type
Journal Article
Genome-wide association study of agronomy and morphology and morphology traits in elite spring wheat Germplasm evaluated under salt stress conditions in Egypt
Authors:
Shaimaa M. Ahmed
Kord A. Maimona, Hafez M. Rehab, Momtaz A. Osama, Amer N. Mohamed, Mobarak H. Mohamed, Alsamman M.Alsamman, Aladdin Hamwieh, Wuletaw Tadesse

Soil salinity is a long-term issue that needs addressing by developing tolerant cultivars. Today,
genome-wide association studies (GWAS) is a preferred tool for quantitative trail loci detection for
different traits. Ten salinity-related attributes in a broad panel of bread wheat were evaluated in
saline field (10 dSm-1 NaCl), at El-Arish, North Sinai, Egypt. We detected genomic loci associated
with salt tolerance using over, 24145 single nucleotide polymorphism. One experiment was
conducted in two seasons; under saline soil conditions of El-Arish, Sinai, where soil ECe was 8.68
and 9.31 dSm-1 in the first and second season, respectively. Ten trait-marker associations were
detected on the 1D, 3A, and 6B chromosomes of bread wheat. For spikes/plant and tillers/plant, seven common SNPs (SNP- BobWhite_c8340_511, SNP- BobWhite_rep_c49102_169, SNPBS00068520_51, SNP- IAAV3900, SNP- Kukri_c34195_357, SNP- Kukri_c80104_809 and SNPwsnp_RFL_Contig429_4978628) were identified on chromosome 3A. For plant height, SNPExcalibur_c91980_139 was identified on chromosome 6B in the two seasons and for spike length,
SNP- D_GA8KES401EYUM8_190 was identified on chromsome 1D. SNP
BobWhite_rep_c49102_169 was previously reported to be linked to germination under salt stress.
BS00068520_51 was found to be associated to thousand kernel weight while the SNPs
Kukri_c34195_357 and Kukri_c80104_809 were associated with flour colour related traits, under
non saline conditions. These SNPs were associated with spikes and tillers/plant in the present study.
The other six SNPs are novel to be found to salinity tolerance related traits.

Citation:
Shaimaa M. Ahmed, Kord A. Maimona, Hafez M. Rehab, Momtaz A. Osama, Amer N. Mohamed, Mobarak H. Mohamed, Alsamman M. Alsamman, Aladdin Hamwieh, Wuletaw Tadesse. (16/11/2021). Genome-wide association study of agronomy and morphology and morphology traits in elite spring wheat Germplasm evaluated under salt stress conditions in Egypt. Plant Cell Biotechnology and Molecular Biology, 22 (65-66), pp. 88-98.
Keywords:
candidate genes
field evaluation
salinity tolerance
genome-wide association mapping
single nucleotide polymorphism markers
triticum aestivum